Insta-Your-Cells: Vote

The voting period for the ISCT 2022-2023 Insta-Your-Cells Photo Challenge is now closed

Prizes

  • The first three prizes include a complimentary registration to ISCT 2023 Paris in addition to:

    • 1st Place: Image showcased on cover of May 2023 Issue of Cytotherapy + a framed keepsake of the May Cytotherapy cover

    • 2nd Place: Image showcased on cover of June 2023 Issue of Cytotherapy

    • 3rd Place: Image showcased on cover of July 2023 Issue of Cytotherapy


Note that all outstanding image submissions are eligible to be featured on the front cover of Cytotherapy (starting with the August 2023 issue)
2022-2023 Insta-Your-Cells Submitted Images

Image 1
This is an image representing the cytoskeleton of a mesenchymal stromal cell at X40 magnification, the green marking shows the actin fibers and the red the microtubules while the blue is a dapi marking the nucleus

2.pngImage 2
These chondrocytes are derived from clinically grown mesenchymal stromal cells (MSCs) which underwent a trilineage assay. Once released for clinical use, the MSCs are infused during infant open-heart surgery to keep brain swelling down during bypass
Image 3
These osteocytes are derived from clinically grown mesenchymal stromal cells (MSCs) which underwent a trilineage assay. Once released for clinical use, the MSCs are infused during infant open-heart surgery to keep brain swelling down during bypass
Image 4
MSCs were cultured in adipogenic differentiation medium, and 20 days later they formed mature adipocytes. Oil-Red-O was used to visualize lipid deposits in the cells, using bright field microscopy (Leica, 10x objective). Broken heart pattern
Image 5
MSCs were cultured in adipogenic differentiation medium, and 20 days later they formed mature adipocytes. Oil-Red-O was used to visualize lipid deposits in the cells, using bright field microscopy (Leica, 10x objective). Strawberry-like looking cells
6.jpgImage 6
Nuclear-Lamin-GFP and cell membrane (magenta) staining of iPSC-derived mature retinal pigment epithelium cells 


Image 7
iPSC-derived retinal pigment epithelium monolayer stained for membrane markers, ZO-1 (green) and Beta-CATENIN (red). Nucleus is stained in blue 
Image 8
Human Adipose derived mesenchymal stromal cells (Actin: yellow, Nucleus: white, vimentine: orange) 
Image 9
Human Adipose derived mesenchymal stromal cells (Actin: green, Nucleus: white, vimentine: pink) 

Image 10
Human adipose-derived mesenchymal stromal cells (actin: blue-green; nucleus: yellow)



Image 11
Genetically modified adult mesenchymal stem cell to up-regulate the age suppressor gene Klotho





Image 12
Soft bone callus µtissue from human periosteum-derived cells, 21 days of differentiation. Collagen fibers in red, actin staining in green, OSX in magenta and nuclei in blue

Image 13
Soft bone callus µtissue from human periosteum-derived cells, 21 days of differentiation. Collagen fibers in red, actin staining in green, OSX in magenta and nuclei in blue

Image 14
Soft bone callus µtissue from human periosteum-derived cells, 14 days of differentiation. Collagen fibers in red, actin staining in green, Ki67 in magenta

Image 15
Human monocytes efferocytose human UC-MSCs. Red: CD14 on Monocytes, Green: MSC labeled with DiO Blue: Nuclei stained with Hoechst




Image 16
A bone marrow stromal cell (nucleus stained with Hoechst 33432 and healthy mitochondria stained with MitoTracker Red). Scale bar = 30micrometer




Image 17
This image shows the dancing stem cells. The human mesenchymal stem cell behavior was changed during culture on the designated surface with nano-topographical modification. Immunofluorescence staining towards F-actin (green) and nuclear lamin (red) 

Image 18
Half of the heart. The slicing confocal image of differentiated hMSCs towards cardiomyocyte. The aggregate cells of hMSCs during differentiation towards cardiomyogenic commitment were observed using F-actin (green) and nuclear lamin (red) markers

Image 19
The aggregate. Cell aggregate of hMSCs during commitments towards cardiomyocyte under confocal observation






Image 20
Adipo- differentiated human MSCs treated with Conjugated linoleic acid, CLA. The cells have been stained for mitochondria, lipid droplets, and DNA. Imaging is done using LSM 880, Airyscan detection at 100 x magnification 

Image 21
Osteo differentiated human MSCs stained for RUNX2, (transcription factor) and actin filaments. Imaged using the LSM 880 from Zeiss at 20x magnification on the AiryScan detector

Image 22
Adipo- differentiated human MSCs treated with Rosiglitazone, RSG. The cells have been stained for mitochondria, lipid droplets, and DNA. Imaging is done using LSM 880, Airyscan detection at 100 x magnification


Image 23
Osteoblast differentiated from MSCs stained for protein OPN, DNA and Actin. Imaging is done using LSM 880, Airyscan detection at 100 x magnification




Image 24
Adipocytes differentiated from human MSCs treated with the drug Pioglitazone. The cells were stained for lipid droplets with LipiTox Green dye. Imaging is done using LSM 880, Airyscan detection at 20 x magnification along with DIC imaging

Image 25
Osteoblast differentiated from human ADSCs stained for RUNX2(transcription factor) and actin filaments. Imaged using the LSM 880 from Zeiss at 20x magnification on the AiryScan detector 
4ccb4365-59db-41b2-b32e-f5f78c4b9dfaImage 26
Murine Osteoclast differentiated from RAW264.7 cells under the presence of the drug pioglitazone. The cells were stained for nuclei and Actin. Imaging is done using Cell Discoverer7 at 40 x magnification



Image 27
Murine Osteoclast differentiated from RAW264.7 cells under the presence of the drug pioglitazone. The cells were stained for nuclei and Actin. Imaging is done using Cell Discoverer7 at 40 x magnification and cropped 








Image 28
Adipo- differentiated human MSCs treated with CLA. The cells have been stained for mitochondria, lipid droplets, and DNA. Imaging is done using LSM 880, Airyscan detection at 100 x magnification 

Image 29
T-cell acute lymphoblastic leukemia blasts "in love" analyzed by flow cytometry 






Image 30
Maximum intensity projection of rods and cones stained in the eye of zebrafish larva along with nuclei and actin



Image 31
Bovine adult adipose tissue-derived mesenchymal stromal cells showing adipogenic differentiation as visualized by immunofluorescence. Image showing fat droplets (red: AdipoRed) and nuclei (Hoechst)

Image 32
CD11b expression on the surface of bovine peripheral blood-derived leukocytes as visualized by immunofluorescence. Image showing CD11b expression (green: FITC) and nuclei (blue: Hoechst)




Image 33
Bovine adult adipose tissue-derived mesenchymal stromal cells showing adipogenic differentiation. Image showing fat droplets (red: Oil Red O) and nuclei (purple: hematoxylin)





Image 34
Bovine fetal adipose tissue-derived mesenchymal stromal cells showing spontaneous osteogenic differentiation. Image showing calcium deposits (red: Alizarin Red S)




Image 35
Bovine fetal adipose tissue-derived mesenchymal stromal cells showing chondrogenic differentiation. Image showing glycosaminoglycan content (blue: Alcian Blue) and nuclei (red: Nuclear Fast Red)

Image 36
Confocal image of iPSC-derived astrocytes stained with GFAP on week 11 of differentiation under 20x 



Image 37
Confocal image of iPSC-derived astrocytes stained with GFAP on week 11 of differentiation under 40X 



Image 38
Retinal pigmented epithilium (RPE) cells derived from hESC. On red, HLA-I (a marker for the human major histocompatibility complex one), on green, ZO-1 (a marker for tight junctions, highly expressed on RPE cells), and on blue, DAPI (nuclear marker)


Image 39
iPS-derived neural microtissue stained for dopaminergic neurons (TH marker, false color depth encoding) obtained after 24-day neurodifferentiation in bioreactor with the C-Stem™ technology

Image 40
MSC in the pancreas











Image 41
This image shown here are iPSCs stained by Phalloidin (red), alpha-tubulin (green), gammaH2AX(white) and DAPI (blue). The mitotic spindles were nicely labelled by alpha-tubulin, and look like fireworks 


Image 42
This image shown here is a differentiation culture of iPSCs derived dopamine neurons at day 80 of differentiation. TH is shown in green and DAPI is shown in blue. At d80, dopamine neurons develop elaborate networks, seen by the extensive TH neurites 

Image 43
This image shown here is a differentiation culture of iPSCs derived GABA neurons at day 70 of differentiation. SST is shown in red and MAP2 in green. SST positive GABA neurons are interneurons, have elaborate processes without long projections 


Image 44
PDAC microenvironment from a syngeneic mouse model captured using morphology markers for tumor cells (PanCK, green), host hematopoietic cells (CD45.2, violet), and anti-mesothelin-CAR T cells (RNAscope, red) for GeoMx® DSP spatial characterization 


Image 45
Syngeneic PDAC tumor after combinatory treatment with anti-mesothelin CAR T cells and agonistic immunostimulatory Ab captured using RNAscope probes for tumor cells (mesothelin, green), T cells (CD3, red), and anti-mesothelin-CAR T cells (CAR, pink) 

Image 46
Formation of vascular smooth muscle cells within embryoid body stained with alpha-smooth muscle actin (red) counterstained with DAPI (blue) 

Image 47
Human endothelial cells subjected to cytotoxic stressor stained with CD31 (white), actin filaments (red) and Dapi (blue)


Image 48
Human iPSC differentiated endothelial cells. Peri-nuclear Von Willebrand Factor stained against alpha-tubulin (purple) and counterstained with DAPI (blue) 

Image 49
Human endothelial cells stained with VE-Cadherin (red), alpha-tubulin (white) and nucleus counterstained with DAPI (blue)



Image 50
Human iPSC-derived endothelial cells characterized by expression of CD31 (white) stained against Actin (red) and nucleus counterstained with DAPI (blue) 


Image 51
Cobblestone-like human endothelial cells labelled with peri-nuclear Von Willebrand Factor (yellow), alpha-tubulin (magenta) and nucleus counterstained with DAPI (blue)

Image 52
Human iPSC-derived lung epithelial cells induced to undergo epithelial - mesenchymal transition. Cells were labelled with vimentin (green), EpCAM (orange), and the nucleus counterstained with DAPI (blue)



Image 53
Human pulmonary epithelial cells enriched with pre-messenger RNA splicing factors (SUMO5 - green); cytoskeleton (alpha tubulin - red), and nuclei (blue)





Image 54
Self-assembling human cardiosphere. Cardiac progenitors display nuclear expression of NKX2.5 (turquoise); mitochondria (dark pink), and actin cytoskeleton (orange) 

Image 55
Image of inflamed tonsils tissue stained with CD4 cells , nuceli and cytokeratin imaged using cell discoverer 7 with a 20x objective

Image 56
Micromass of human Mesenchymal Stromal Cells under chondrocyte differentiation evaluated by Alcian Blue staining 




Image 57
Micromass of human Mesenchymal Stromal Cells under chondrocyte differentiation evaluated by Alcian Blue staining 




Image 58
Adipocytes from human Mesenchymal Stromal Cells differentiation 





Image 59
Ultrastructure of a bovine adipose tissue-derived mesenchymal stromal cell producing fat droplets during adipogenic differentiation as visualized by scanning electron microscopy (Food Structure and Function, Prof. Koen Dewettinck) 

Image 60
Heart-like droplet among undifferentiated Mesenchymal stem cells. Peace, Love, Hope under the microscope

Image 61
Injured skeletal muscle tissue with embryonic Myosin Heavy Chain (Red), DAPI (Blue), and laminin (Green) staining. Central nuclei and Myosin Heavy Chain positive fibres represent the regenerating muscle


Image 62
Mitochondrial energizing highway. A mesenchymal stem cell (MitoTracker Red) transfers mitochondria to a metabolically stressed cell (CellTracker Green) by a nanotube internalized in the recipient cell cytoplasm. Confocal image

Image 63
This image is DTZ-stained human pancreatic islets. The human pancreatic islets were isolated from a cadaveric donor





Image 64
This is an image of human amniotic epithelial cells isolated from a human placenta and cultured for one week


Image 65
This is FDA/PI stained human islets and human amniotic epithelial cells. These cocultured cells were subject to the FDA/PI stain for viability test

Image 66
New Life - A smooth muscle cell escaping out from digested tissue extracellular matrix to start a new life





Image 67
Gingival mesenchymal stem cells isolated from Human Gingival tissue




Image 68
Human umbilical vein endothelial cells derived from umbilical cord 





Image 69
Image of a dental pulp mesenchymal stem cell (DPSC) transduced with lentivirus expressing GFP