iPSC Culture and Application in Cell and Gene Therapy

iPSC Culture and Application in Cell and Gene Therapy

This 2-hour on-demand course and 1-hour live-virtual discussion provides a comprehensive foundation in the reprogramming of somatic cells into induced pluripotent stem cells (iPSCs), a crucial technique in regenerative medicine and cell therapy. Designed by leading academic and industry experts, the course blends self-paced modules with interactive live-virtual discussions, offering both theoretical insights and practical guidance.

You will explore the entire reprogramming process, from the key steps involved to the specific workflow considerations based on the starting material. Gain an in-depth understanding of both viral and non-viral reprogramming methods and learn to distinguish between undifferentiated and differentiated iPSC colonies. The course also covers essential assays to evaluate iPSC quality and their readiness for therapeutic use.

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Hybrid 
(self-paced with live-virtual Q&A)
Coming Soon in Spring 2025
COURSE CONTENT

Module 1: Reprogramming Somatic Cells to iPSCs

  • Steps involved in reprogramming somatic cells into induced pluripotent stem cells (iPSCs).
  • Workflow considerations based on the starting material used.
  • Key differences between viral and non-viral reprogramming methods.
  • Morphological features of iPSC colonies ready for selection and differentiation.
  • Characteristics of undifferentiated versus differentiated iPSC colonies.
  • Assays to evaluate iPSC quality.

Module 2: Quality by Design (QbD) in Culturing iPSCs for Cell Therapy

  • Functional characteristics of iPSCs as starting material for cell therapies.
  • Factors influencing pluripotency and quality of iPSCs.
  • Comparison between traditional and QbD-based automated cell manufacturing processes.
  • Key in-process parameters for monitoring iPSC quality during manufacturing.

Module 3: Development of Multi-Engineered, iPSC-Derived Therapeutic Products

  • Advantages of allogeneic cell therapy manufacturing.
  • Benefits of using iPSCs over immune cell types in cell therapies.
  • Differences in manufacturing workflows: iPSCs vs. immune cell types.
  • Key challenges, risk assessments, and regulatory considerations in using iPSCs for therapeutic applications.
  • The use of iPSC differentiation processes for therapy development.
LEARNING OUTCOMES
  • Describe the biology, key features, and potential applications of human pluripotent stem cells (hPSCs).
  • Understand and outline the workflow for selecting and expanding iPSC.
  • Identify and describe the critical quality attributes (CQAs) of hPSCs and their significance.
  • Demonstrate knowledge of the processes involved in hPSC manufacturing.
  • Recognize the use of iPSCs.
DESIGNED FOR:

Researchers, lab professionals, and process development engineers with cell culture experience, seeking to master best practices for culturing iPSCs and their application in cell therapies.

ACCREDITED BY:

16 CMLE Hours

UPON COMPLETION, YOU WILL RECEIVE:
  • 16 CMLE Credits
  • Certificate of Course Completion from ISCT

Note: Completing all training modules and passing the quizzes is mandatory to receive a course completion certificate. 

 PLANNING COMMITTEE 

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