iPSC Culture and Application in Cell and Gene Therapy

COURSE OVERVIEW

Gain fundamental knowledge in reprogramming somatic cells into induced pluripotent stem cells (iPSCs). Through a combination of self-paced modules and interactive live-virtual discussions, you'll learn from leading global experts how to evaluate the quality and key characteristics of iPSCs, ensuring their suitability for advanced research and therapeutic applications.

COURSE CONTENT

Module 1: Reprogramming Somatic Cells to iPSCs

• Steps involved in reprogramming somatic cells into induced pluripotent stem cells (iPSCs).
• Workflow considerations based on the starting material used.
• Key differences between viral and non-viral reprogramming methods.
• Morphological features of iPSC colonies ready for selection and differentiation.
• Characteristics of undifferentiated versus differentiated iPSC colonies.
• Assays to evaluate iPSC quality.

Module 2: Quality by Design (QbD) in Culturing iPSCs for Cell Therapy

• Functional characteristics of iPSCs as starting material for cell therapies.
• Factors influencing pluripotency and quality of iPSCs.
• Comparison between traditional and QbD-based automated cell manufacturing processes.
• Key in-process parameters for monitoring iPSC quality during manufacturing.

Module 3: Development of Multi-Engineered, iPSC-Derived Therapeutic Products

• Advantages of allogeneic cell therapy manufacturing.
• Benefits of using iPSCs over immune cell types in cell therapies.
• Differences in manufacturing workflows: iPSCs vs. immune cell types.
• Key challenges, risk assessments, and regulatory considerations in using iPSCs for therapeutic applications.
• The use of iPSC differentiation processes for therapy development.

LEARNING OUTCOMES

• Describe the biology, key features, and potential applications of human pluripotent stem cells (hPSCs).
• Understand and outline the workflow for selecting and expanding iPSC.
• Identify and describe the critical quality attributes (CQAs) of hPSCs and their significance.
• Demonstrate knowledge of the processes involved in hPSC manufacturing.
• Recognize the use of iPSCs.

DESIGNED FOR:

• Professionals seeking to enhance their expertise in iPSC technology, including its applications and advancements.
• CGT professionals and clinical researchers working in regenerative medicine or developing therapies and products based on iPSCs.

UPON COMPLETION, YOU WILL RECEIVE:

• A certificate of course completion from ISCT