iPSC Culture and Application in Cell and Gene Therapy

Self-Paced Online Course
2 hours of self-paced,
on-demand instructional modules.
Live Virtual Session
1-hour live-virtual expert panel discussion on April 13, 2025 (16:00 PT / 19:00 ET / 23:00 GMT).

iPSC Culture and Application in Cell and Gene Therapy

This 2-hour on-demand course and 1-hour live-virtual discussion provides a comprehensive foundation in the reprogramming of somatic cells into induced pluripotent stem cells (iPSCs), a crucial technique in regenerative medicine and cell therapy. Designed by leading academic and industry experts, the course blends self-paced modules with interactive live-virtual discussions, offering both theoretical insights and practical guidance. You will explore the entire reprogramming process, from the key steps involved to the specific workflow considerations based on the starting material. Gain an in-depth understanding of both viral and non-viral reprogramming methods and learn to distinguish between undifferentiated and differentiated iPSC colonies. The course also covers essential assays to evaluate iPSC quality and their readiness for therapeutic use.

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Based on Past Participant Survey Results
89%
Rated the speakers as knowledgeable and well organized
89%
Would recommend the webinar to a colleague
78%
Said the webinar met their needs

  • Cell Therapy Process Development: Understand key factors for designing cell therapy processes suitable for GMP translation.
  • Emerging Technologies: Evaluate and integrate new technologies and equipment for CGT advancement.
  • Standardized Methods & Assays: Apply consistent methodologies for establishing assays and foundational CGT workflows.
  • Immune & Stem-Cell Frameworks: Implement standardized processes for immune- and stem-cell–based therapies.
  • Gene Modification Techniques: Gain hands-on experience modifying primary and stem cells.
  • Artificial Intelligence in CGT: Understand how AI supports and enhances CGT processing and analysis.
  • Understand GMP & Good Documentation Practices (GDP).
  • Learn workflow for cell handling, processing & release.
  • Practice aseptic technique, flow cytometry & transduction.
  • Review batch records, deviations & CAPA.
  • Develop competency in harvest, cryopreservation & assay release.

The Resource Hub provides supporting materials to help reinforce learning before, during, and after the course.

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ELIGIBILITY REQUIREMENTS
  • None

This course is structured into the following modules:

Module 1: Reprogramming Somatic Cells to iPSCs

  • Steps involved in reprogramming somatic cells into induced pluripotent stem cells (iPSCs)
  • Workflow considerations based on starting material
  • Key differences between viral and non-viral reprogramming methods
  • Morphological features of iPSC colonies ready for selection and differentiation
  • Characteristics of undifferentiated vs. differentiated iPSC colonies
  • Assays used to evaluate iPSC quality

Module 2: Quality by Design (QbD) in Culturing iPSCs

  • Functional characteristics of iPSCs as starting material for cell therapies
  • Factors influencing pluripotency and iPSC quality
  • Comparison between traditional and QbD-based automated manufacturing approaches
  • Key in-process parameters for monitoring iPSC quality

Module 3: iPSC-Derived Therapeutic Products

  • Advantages of allogeneic cell therapy manufacturing
  • Benefits of using iPSCs compared to immune cell types
  • Workflow differences between iPSCs and immune cell-based manufacturing
  • Key challenges, risk assessments, and regulatory considerations
  • iPSC differentiation processes for therapy development
  • Describe the biology, key features, and applications of human pluripotent stem cells (hPSCs).
  • Understand and outline the workflow for selecting and expanding iPSCs.
  • Identify and describe critical quality attributes (CQAs) of hPSCs.
  • Demonstrate knowledge of processes involved in hPSC manufacturing.
Designed For

Researchers, lab professionals, and process development engineers with cell culture experience who are seeking to master best practices for culturing iPSCs and applying them in cell therapy manufacturing.

ELIGIBILITY REQUIREMENTS
  • None

Pricing (USD)

Training Course Only ISCT Members Non-Members (includes 1-year e-membership)
Student $250 $305
Resident / Fellow / Postdoc $250 $315
Technologist / Pharmacist / Nurse Practitioner / Nurse $300 $430
Academic¹ $300 $485
Industry $390 $575
Tier 1 reduced rate² $210 $270
Tier 2 reduced rate² $210 $297.50
15% Group Registration Discount available for 5+ registrants from the same institution

Group Registration Discount:

1 Contact ISCT Head Office for Academic pricing codes.

2 Tier 1 & Tier 2 countries listed here.

Headshot of Shin Kawamata

Shin Kawamata, MD, PhD

CEO, Cyto-Facto (Japan)

“To unlock the full clinical potential of iPSCs, it’s critical to understand not just their biology, but also how to maintain them properly, scale them up efficiently, and apply best practices across the entire workflow. This course brings all those elements together for CGT professionals aiming to translate iPSC-based therapies into the clinic.”

Headshot of Lise Munsie

Lise Munsie, PhD

VP, Process Development, BlueRock (USA)

“Maintaining iPSC quality starts with understanding the fundamentals, from colony morphology to genomic stability. This training gives learners the confidence to navigate the complexity of iPSC systems with clarity and scientific rigor.”

Headshot of Bruno Marques

Bruno Marques, PhD

VP, Technical Development, Century (USA)

“Establishing a robust and scalable iPSC process requires more than just technical skill, it demands a clear understanding of regulatory expectations and clinical readiness. This course provides that foundation, giving participants a real-world view of what it takes to bring iPSC-based products to patients.”

UPON COMPLETION, YOU WILL RECEIVE:
  • 3 CMLE Credits
  • Certificate of Course Completion from ISCT

Note: Completing all training modules and passing the quizzes is mandatory to receive a course completion certificate.

ACCREDITED BY:
3 CMLE Hours
What Past Participants Are Saying

“The speakers delivered the material in a clear and well-structured way, making complex iPSC concepts easy to follow. I especially appreciated how the sessions connected theory to real research applications and clinical translation.”

Amanda K.

“Great speakers with diverse backgrounds from both academia and industry. The discussions on challenges of bringing iPSC therapies to clinical trials were particularly valuable and provided practical insight I can apply to my own work.”

Olivia G.

“The webinar content was relevant, informative, and directly applicable to my field. It strengthened my confidence in implementing what I learned and I would recommend this training to colleagues interested in cell therapy manufacturing and applications.”

Christina L.

Planning Committee

Shin Kawamata
Shin Kawamata
MD, PhD
ISCT Asia Regional Secretary
ISCT iPSC Committee Co-Chair
Cyto-Facto
Japan
Bruno Marques
Bruno Marques
PhD
ISCT iPSC Committee
Century Therapeutics
United States
Lise Munsie
Lise Munsie
PhD
ISCT iPSC Committee
BlueRock Therapeutics
Canada

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